Web supplement to
"The Chemical Genomic Portrait of Yeast: Uncovering a Phenotype for All Genes"

Maureen E. Hillenmeyer, Eula Fung, Jan Wildenhain, Sarah E. Pierce, Shawn Hoon, William Lee, Michael Proctor, Bob P. St. Onge, Mike Tyers, Daphne Koller, Russ B. Altman, Ronald W. Davis, Corey Nislow, and Guri Giaever.

DNA Damage Response

Abstract

Genetics aims to understand the relation between genotype and phenotype. However, because complete deletion of most yeast genes (~80%) has no obvious phenotypic consequence in rich medium, it is difficult to study their functions. To uncover phenotypes for this nonessential fraction of the genome, we performed 1144 chemical genomic assays on the yeast whole-genome heterozygous and homozygous deletion collections and quantified the growth fitness of each deletion strain in the presence of chemical or environmental stress conditions. We found that 97% of gene deletions exhibited a measurable growth phenotype, suggesting that nearly all genes are essential for optimal growth in at least one condition.

Interactive website: FitDB

For a graphical interface to the chemogenomic fitness data, visit FitDB or type a query in the box on the left.





Inquiries can be addressed to guri.giaever@utoronto.ca.