Web supplement to
"A unique and universal molecular barcode array"

Files

• TAG4_probe_info_readme.txt
• TAG4_probe_info.txt
• SupplTable1_repaired_tag_info.xls
• Genflex_TAG4_wash_protocol.MAC

Analysis scripts

These Perl scripts implement the data analysis methods described in the paper.

• raw_file_maker.pl -- masks outliers and average unmasked replicates.
• normalize_data.pl -- normalizes data using quantile normalization.
• fitness_profile.pl -- compares a set of treatment data to a control set.
  
  
• support files -- files describing the deletion strains and tags on the Tag4 chip. These files are needed by the scripts and should be put in a directory called "files" located in the same directory as the scripts.

To run these scripts, you will need to have Perl 5 installed. Run each script with no arguments to get a short usage blurb. Each script has an option to create PNG images, but you will need to have the GD perl module installed for this option to work. Also, the "fitness_profile.pl" script has an option to calculate p-values, but this option requires the Math::CDF perl module.

Note: GD.pm can be tedious to install manually due to its many dependencies. If you are using Mac OS X, it is easy to install by using fink or MacPorts. If you are using Windows with ActivePerl, see their note on GD installation here.